The detection of astrovirus in sludge biosolids using an integrated cell culture nested PCR technique

Citation
Cd. Chapron et al., The detection of astrovirus in sludge biosolids using an integrated cell culture nested PCR technique, J APPL MICR, 89(1), 2000, pp. 11-15
Citations number
30
Categorie Soggetti
Biology,Microbiology
Journal title
JOURNAL OF APPLIED MICROBIOLOGY
ISSN journal
13645072 → ACNP
Volume
89
Issue
1
Year of publication
2000
Pages
11 - 15
Database
ISI
SICI code
1364-5072(200007)89:1<11:TDOAIS>2.0.ZU;2-Y
Abstract
The work presented here demonstrates the utility of the integrated cell cul ture-reverse transcriptase-polymerase chain reaction( ICC-RT-PCR) coupled w ith nested PCR to detect human astroviruses and enteroviruses in sludge bio solids. Viruses were concentrated by beef extract elution and organic flocc ulation prior to analysis by a plaque assay and ICC-RT-PCR. Astroviruses we re detected in all but one sample and all of the samples were positive for enteroviruses. We have demonstrated the prevalence and frequency of astrovi rus in sludge and validated the ICC-RT-PCR/nested PCR technique as a useful tool to detect viruses in sludge.