Sequencing, cloning, and high-level expression of the pfp gene, encoding aPPi-dependent phosphofructokinase from the extremely thermophilic eubacterium Dictyoglomus thermophilum

Authors
Ding, YHR Ronimus, RS Morgan, HW
Citation
Yhr. Ding et al., Sequencing, cloning, and high-level expression of the pfp gene, encoding aPPi-dependent phosphofructokinase from the extremely thermophilic eubacterium Dictyoglomus thermophilum, J BACT, 182(16), 2000, pp. 4661-4666
Citations number
28
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
00219193 → ACNP
Volume
182
Issue
16
Year of publication
2000
Pages
4661 - 4666
Database
ISI
SICI code
0021-9193(200008)182:16<4661:SCAHEO>2.0.ZU;2-G
Abstract
The sequencing, cloning, and expression of the pfp gene from Dictyoglomus t hermophilum, which consists of 1,041 bp and encodes a pyrophosphate-depende nt phosphofructokinase, are described. A phylogenetic analysis indicates th at the enzyme is closely related to the pyrophosphate-dependent enzyme from Thermoproteus tenax. The recombinant and native enzymes share a high degre e of similarity for most properties examined.