Conformational changes of oligomeric particle of GroEL chaperone from
E. coli in solution were studied, which proceed during its denaturatio
n upon the action of elevated urea concentration, temperature, and ext
remal pH values by the methods of CD, light scattering, scanning micro
calorimetry, hydrophobic probe binding, and ATPase activity measuremen
ts. The ranges of changing the external conditions, within which GroEL
retains its structure and functions, were determined. Denaturation tr
ansitions were found to be cooperative, pronounced, and irreversible.
In the pH range from 6.0 to 9.6, the three-step change of the ATPase a
ctivity of GroEL was shown to occur with half-transition pH(1/2) of 6.
3, 8.5, and 9.3. It does not result in any essential structural change
s and is probably associated with a protonation/deprotonation of amino
acid residues important for the GroEL ATPase activity.