RECOMBINANT HIS(6)-TAGGED DNA-POLYMERASE FORM THERMUS-THERMOPHILUS WITH THE REVERSE-TRANSCRIPTASE ACTIVITY

Authors
SMIRNOV YV CHAKHMAKHCHEVA OG EFIMOV VA
Citation
Yv. Smirnov et al., RECOMBINANT HIS(6)-TAGGED DNA-POLYMERASE FORM THERMUS-THERMOPHILUS WITH THE REVERSE-TRANSCRIPTASE ACTIVITY, Bioorganiceskaa himia, 23(4), 1997, pp. 257-261
Citations number
11
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Bioorganiceskaa himia → ACNP
ISSN journal
01323423
Volume
23
Issue
4
Year of publication
1997
Pages
257 - 261
Database
ISI
SICI code
0132-3423(1997)23:4<257:RHDFTW>2.0.ZU;2-8
Abstract
The Tth DNA polymerase genes from the thermophilic bacterium Thermus t hermophilus (strains HB-8 and Tt-111) were cloned and expressed in Esc herichia coil cells. The target protein contained a polyhistidine tag at the N-terminus that allowed its single-stage isolation by affinity chromatography on Ni-NTA-agarose. The isolated enzyme displayed both h igh DNA polymerase and reverse transcriptase activities.