A novel class of highly potent and selective A(1) adenosine antagonists: Structure-affinity profile of a series of 1,8-naphthyridine derivatives

Citation
Pl. Ferrarini et al., A novel class of highly potent and selective A(1) adenosine antagonists: Structure-affinity profile of a series of 1,8-naphthyridine derivatives, J MED CHEM, 43(15), 2000, pp. 2814-2823
Citations number
36
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF MEDICINAL CHEMISTRY
ISSN journal
00222623 → ACNP
Volume
43
Issue
15
Year of publication
2000
Pages
2814 - 2823
Database
ISI
SICI code
0022-2623(20000727)43:15<2814:ANCOHP>2.0.ZU;2-E
Abstract
A series of 1,8-naphthyridine derivatives (12-36), bearing a phenyl group i n position 2 and various substituents in positions 4 and 7, were synthesize d in an attempt to obtain potent, selective antagonists for the A(1) adenos ine receptor subtype. The compounds were tested to evaluate their affinity for A(1) compared with A(2A) and A(3) adenosine receptor subtypes. In bindi ng studies in bovine brain cortical membranes, most of the compounds showed an affinity for A(1) receptors in the low nanomolar range and two in the s ubnanomolar range with an interesting degree of A(1) versus A(2A) and A(3) selectivity. Comparison of the 4-substituted derivatives indicated that 4-O H substitution, with a 4-quinoid structure, causes an increase in the A(1) and A(2A) affinity and generally also in A(1) selectivity. The kind of subs titution in position 7 can greatly modulate the affinity: the most interest ing substituents in this position seemed to be electron-withdrawing groups; in particular the 7-chloronaphthyridine 25d showed a remarkable selectivit y (A(2A)/A(1) ratio of 670, A(3)/A(1) ratio of 14 000) associated with a hi gher A(1) affinity (K-i = 0.15 nM). NMR studies on these compounds 12-36 in dicated that the 4-OH-substituted ones prefer the tautomer in which the oxy gen in position 4 is in the quinoid form and the nitrogen in position 1 is protonated. Theoretical calculations are in agreement with the NMR data.