E. Hammarberg et al., Synthesis of novel 5-substituted 3-amino-3,4-dihydro-2H-1-benzopyran derivatives and their interactions with the 5-HT1A receptor, J MED CHEM, 43(15), 2000, pp. 2837-2850
A series of new enantiomerically pure 3-amino-3,4-dihydro-2H-1-benzopyrans
(3-aminochromans) has been synthesized from (R)- and (S)-5-methoxy-3-amino-
3,4-dihydro-2H-1-benzopyran The absolute configuration of the respective (R
)- and (S)-enantiomers was deduced from X-ray crystallography of (R)-3-(N-i
sopropylamino)-5-methoxy-3,4-dihydro-2H-1-benzopyran, (R)-9a. Various 5-sub
stituents were introduced via palladium-catalyzed carbonylation of N-substi
tuted 3-amino-5-trifluoromethanesulfonyloxy-3,4-dihydro- 2H-1-benzopyran. T
he effect of N- and 5-substitution on affinity for the 5-HT1A receptor was
evaluated in competition experiments using rat hippocampal membranes and [H
-3]8-OH-DPAT as radioligand. Selected compounds were also tested for their
affinity to the D-1 (rat striatum), D-2 (rat striatum), D-2A (human cloned)
, and 5-HT2A (rat cortex) receptors. The intrinsic activity of the compound
s was evaluated by measuring their effect on VIP-stimulated cAMP production
in GH(4)ZD10 cells stably transfected with the 5-HT1A receptor. High-affin
ity compounds with high selectivity for the 5-HT1A receptor were found amon
g structures substituted with carboxylate esters, amides, and ketones in th
e 5-position. Primary and secondary amines bound with lower affinity than t
ertiary amines. Larger substituents were well-tolerated by the receptor, bu
t the smaller N-ethyl-N-isopropyl bound with lower affinity. Generally, the
(R)-enantiomers displayed higher affinity for the 5-HT1A receptor than the
corresponding (S)-enantiomers. In the present series of compounds, both fu
ll and partial agonists mere found.