Silver stained polyacrylamide gels and fluorescence-based automated capillary electrophoresis for detection of amplified fragment length polymorphismpatterns obtained from white-rot fungi in the genus Trametes

Silver stained denaturing polyacrylamide gels (PAGEs) and fluorescent denat uring automated capillary electrophoresis (CE) were used to detect amplifie d fragment length polymorphism (AFLP) patterns obtained from white-rot fung i belonging to the genus Trametes. AFLP fingerprinting detected by the fluo rescence-based method as well as by silver staining showed a high discrimin atory power in differentiating nine strains of Trametes ochracea, nine stra ins of Trametes hirsuta and ten isolates of Trametes versicolor. UPGMA dend rograms derived from fluorescently labelled and silver stained AFLP pattern s were similar, but a few differences were detected especially in the clust ering of T. ochracea and T. hirsuta strains. Compared to silver-stained AFL P, detection of fluorescent AFLP was fast, reliable and easy to perform and it facilitated surveying with a computerized analysis system. Fluorescent CE seems to be well suited for studying similarity between Trametes species . (C) 2000 Elsevier Science B.V. All rights reserved.