The irradiation of large samples of biological material in a high neutron f
lux enables the extention of trace element analysis into the ng to pg range
, but raises several problems as gamma heating and radiolysis degrade the s
amples. Gamma heating increases the pressure to a point where either a very
large irradiation container is necessary or a container which can endure t
he high pressure. Several methods to overcome these problems have been publ
ished. The irradiation of open samples was performed as an alternative to t
hese methods. Temperature effects on biological material during irradiation
in a high and in a low neutron flux were compared. As an example, wheat wa
s irradiated in open containers allowing simultaneous temperature measureme
nt in the sample during irradiation.