Subunits of neurosteroid sulfatase from bovine brain

Authors
Park, IH Han, BK Baek, JH Ryu, YW Park, YH Jo, DH
Citation
Ih. Park et al., Subunits of neurosteroid sulfatase from bovine brain, J STEROID B, 73(3-4), 2000, pp. 135-139
Citations number
24
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
ISSN journal
09600760 → ACNP
Volume
73
Issue
3-4
Year of publication
2000
Pages
135 - 139
Database
ISI
SICI code
0960-0760(200006)73:3-4<135:SONSFB>2.0.ZU;2-M
Abstract
We have purified the neurosteroid sulfatase (NSS) from Triton X-100 solubil ized microsomes of bovine brain about 100-fold. The purified enzyme is comp osed of two catalytic units (MW: 57 kDa) and two regulatory units (MW: 38 k Da), making it an alpha(2)beta(2) heterotetramer, whose apparent molecular weight was 180 kDa by gel filtration in the presence of Triton X-100. (C) 2 000 Elsevier Science Ltd. All rights reserved.