Cutaneous window for in vivo observations of organs and angiogenesis

Background and objective. The continuous observation in experimental animal s of internal organs and processes, such as wound healing and angiogenesis, has been achieved using a variety of transparent windows and chambers. Our objective was to develop procedures for these observations using disposabl e material for the window and simple surgical techniques. Methods. For observation of wound healing in the mouse kidney, the kidney w as externalized and a wedge was excised. An oval window of polyvinyl chlori de film was sutured in place in the skin over the wound. The progress of he aling of the wound was observed through the window over 10 days. For observ ation of angiogenesis, adrenocortical cells were transplanted beneath fasci a and muscle and a window was sewn into the skin above the site of transpla ntation. Results. Clear observations could be made using these cutaneous windows ove r the period of the experiments. Healing of a wound in the kidney was photo graphed and measured. The growth of new blood vessels over the site of adre nocortical cell transplantation was observed. Conclusions. Continuous in vivo observations of organs such as the kidney a nd processes such as angiogenesis can be made in experimental animals using this simple technique, (C) 2000 Academic Press.