High frequency of multiple melanomas and breast and pancreas carcinomas inCDKN2A mutation-positive melanoma families

Citation
A. Borg et al., High frequency of multiple melanomas and breast and pancreas carcinomas inCDKN2A mutation-positive melanoma families, J NAT CANC, 92(15), 2000, pp. 1260-1266
Citations number
43
Categorie Soggetti
Oncology,"Onconogenesis & Cancer Research
Volume
92
Issue
15
Year of publication
2000
Pages
1260 - 1266
Database
ISI
SICI code
Abstract
Background: Inherited mutations in the CDKN2A tumor suppressor gene, which encodes the p16(INK4a) protein, and in the cyclin-dependent kinase 4 (CDK4) gene confer susceptibility to cutaneous malignant melanoma, We analyzed fa milies with two or more cases of melanoma for germline mutations in CDKN2A and CDK4 to elucidate the contribution of these gene defects to familial ma lignant melanoma and to the occurrence of other cancer types. Methods: The entire CDKN2A coding region and exon 2 of the CDK4 gene of an affected memb er of each of 52 families from southern Sweden with at least two cases of m elanoma in first- or second-degree relatives were screened for mutations by use of polymerase chain reaction-single-strand conformation polymorphism a nalysis. Statistical tests were two-sided. Results: CDKN2A mutations were f ound in 10 (19%) of the 52 families. Nine families carried an identical alt eration consisting of the insertion of arginine at position 113 of p16(INK4 a), and one carried a missense mutation, in which the valine at position 11 5 was replaced with a glycine. The 113insArg mutant p16(INK4a) was unable t o bind cdk4 and cdk6 in an in vitro binding assay, Six of the 113insArg fam ilies had at least one member with multiple primary melanomas; the 113insAr g families also had a high frequency of other malignancies-in particular, b reast cancer (a total of eight cases compared with the expected 2.1; P = .0 014) and pancreatic cancer (a total of six cases compared with the expected 0.16; P<.0001), Families with breast cancer also had a propensity for mult iple melanomas in females, suggesting that a sex-dependent factor may modif y the phenotypic expression of CDKN2A alterations. Conclusions: Our finding s confirm that the majority of CDKN2A-associated melanoma families in Swede n are due to a single founder mutation, They also show that families with t he CDKN2A 113insArg mutation have an increased risk not only of multiple me lanomas and pancreatic carcinoma but also of breast cancer.