M. Grigorova et al., Spontaneous and X-ray-induced chromosomal aberrations in Werner syndrome cells detected by FISH using chromosome-specific painting probes, MUTAGENESIS, 15(4), 2000, pp. 303-310
Werner syndrome (WS) is a rare autosomal disorder characterized by prematur
e aging exhibiting chromosome instability and predisposition to cancer. Cel
ls derived from WS patients show a variety of constitutionally stable chrom
osomal aberrations as detected by conventional chromosome banding technique
s. We have employed the fluorescence in situ hybridization (FISH) technique
using painting probes for 12 different chromosomes to detect stable chromo
some exchanges in three WS cell lines and three control cell lines. WS cell
lines showed increased frequencies of both stable and unstable chromosome
aberrations detected by FISH and Giemsa staining, respectively. One WS lymp
hoblastoid cell line (KO375) had a 5/12 translocation in all the cells and
similar to 60% of the cells had an additional translocated chromosome 12. A
high frequency of aneuploid cells was found in all the WS cell lines studi
ed. Though WS cells are known to be chromosomally unstable, unlike other ch
romosome instability syndromes they are not sensitive to mutagenic agents.
We studied the frequencies of X-ray-induced chromosomal aberrations in two
WS cell lines and found an similar to 60% increase in the frequencies of fr
agments and no consistent increase in the frequencies of exchanges.