Structure of the DNA binding domain of E-coli SSB bound to ssDNA

Authors
Raghunathan, S Kozlov, AG Lohman, TM Waksman, G
Citation
S. Raghunathan et al., Structure of the DNA binding domain of E-coli SSB bound to ssDNA, NAT ST BIOL, 7(8), 2000, pp. 648-652
Citations number
28
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NATURE STRUCTURAL BIOLOGY
ISSN journal
10728368 → ACNP
Volume
7
Issue
8
Year of publication
2000
Pages
648 - 652
Database
ISI
SICI code
1072-8368(200008)7:8<648:SOTDBD>2.0.ZU;2-H
Abstract
The structure of the homotetrameric DNA binding domain of the single strand ed DNA binding protein from Escherichia coli (Eco SSB) bound to two 35-mer single stranded DNAs was determined to a resolution of 2.8 Angstrom. This s tructure describes the vast network of interactions that results in the ext ensive wrapping of single stranded DNA around the SSB tetramer and suggests a structural basis for its various binding modes.