Structure of the heterodimeric complex between CAD domains of CAD and ICAD

We present here the structure of the complex between the CAD domain of casp ase activated deoxyribonuclease (CAD) and the CAD domain of its inhibitor ( ICAD), determined by nuclear magnetic resonance spectroscopy. The two domai ns adopt a very similar fold, which consists of an alpha-helix and a beta-s heet, and are aligned side by side in the complex. Notably the positive cha rges on the strand beta 2 at one end of the beta-sheet of CAD and negative charges around the opposite end of the beta-sheet of ICAD are paired in the complex. Point mutations of the charged amino acids at this interface, on either CAD or ICAD, prevented formation of the functional CAD-ICAD complex. This implies that the interaction between the CAD domains of CAD and ICAD is an essential step in the correct folding of CAD in the complex.