Glucosamine HCl reduces equine articular cartilage degradation in explant culture

Objective: To determine whether glucosamine inhibits experimentally induced degradation of equine articular cartilage explants. Methods: Articular cartilage was obtained from the antebrachio-carpal and m iddle joints of horses (2-8 years old) killed for reasons unrelated to lame ness. Cartilage discs were harvested from the weight-bearing region of the articular surface and cultured. Media were exchanged daily and the recovere d media stored at 4 degrees C. Explants were maintained in basal media 2 da ys prior to the start of four treatment days. On days 1-4 lipopolysaccharid e (LPS, 10 mu g/ml) or recombinant human interleukin-1 (rhIL-1, 50 ng/ml) w ere added to induce cartilage degradation. To test the potential protective effects of glucosamine, the compound was added in three concentrations (0. 25, 2.5, or 25 mg/ml) and treatments were performed in triplicate. Controls included wells without LPS, rhIL-1 beta, or glucosamine. Nitric oxide, pro teoglycan and matrix metalloproteinases (MMP) released into conditioned med ia and tissue proteoglycan synthesis were measured as indicators of cartila ge metabolism. Results: Maximal nitric oxide production, proteoglycan release, and MMP act ivity were detected 1 day after the addition of LPS or rhIL-1 beta to the m edia. The addition of 25 mg/ml of glucosamine prevented the increase in nit ric oxide production, proteoglycan release and MMP activity induced by LPS or rhIL-1. Conclusions: These data indicate that glucosamine can prevent experimentall y induced cartilage degradation in vitro. (C) 2000 OsteoArthritis Research Society International.