Thrombin stimulates pertussis toxin-sensitivity and -insensitive GTPase activities and ADP-Ribosylation of G(i) in human neuroblastoma SH-EP

Authors
Ogino, Y Sakamoto, Y Kinouchi, T Shimizu, N
Citation
Y. Ogino et al., Thrombin stimulates pertussis toxin-sensitivity and -insensitive GTPase activities and ADP-Ribosylation of G(i) in human neuroblastoma SH-EP, PHARMACOL, 61(1), 2000, pp. 11-13
Citations number
8
Categorie Soggetti
Pharmacology & Toxicology
Journal title
PHARMACOLOGY
ISSN journal
00317012 → ACNP
Volume
61
Issue
1
Year of publication
2000
Pages
11 - 13
Database
ISI
SICI code
0031-7012(2000)61:1<11:TSPTA->2.0.ZU;2-7
Abstract
Kinetic interaction between thrombin receptor and G proteins was investigat ed in human epithelial neuroblastoma cell line, SH-EP. In these cells, both alpha-thrombin and SFLLRNP (one-letter amino-acid code) stimulated GTPase activity and enhanced cholera toxin-catalyzed ADP-ribosylation of G(i2) in a concentration-dependent manner. Basal GTPase activity was attenuated by p ertussis toxin treatment by 35%, however, agonist stimulation was preserved significantly, These results together indicated that thrombin receptor sim ultaneously activates G(i2) and PTX-insensitive G protein(s). Copyright (C) 2000 S. Karger AG, Basel.