A TRANSCRIPT MAP ENCOMPASSING THE MULTIPLE ENDOCRINE NEOPLASIA TYPE-1(MEN1) LOCUS ON CHROMOSOME 11Q13

A transcription map of a 1200-kb region encompassing the MEN1 locus wa s constructed by direct cDNA selection and mapping ESTs. A total of 29 genes were mapped. Ten transcripts were identified by cDNA selection of a focused 300-kb genomic region telomeric to the MEN1 consensus reg ion. Since many of the sequences cloned by cDNA selection also identif ied ESTs from the region, 19 additional RH-mapped ESTs were mapped to the entire contig region by CR amplification of genomic clones. Nine k nown genes, 2 putative human homologues to mouse genes, and is novel t ranscripts map to the region. Transcripts that map to the MEN1 interva l PYGM-D11S449 include SGC35223, IB1256, AA147620, ZFM1, FAU, and CAPN 1. The latter 3 known genes have already been excluded as candidate ME N1 genes. The 2 putative human homologues of mouse genes Ltbp2 and Spa -1 may be candidate tumor suppressor genes, but they map telomeric to D11S449. Although both of these genes map outside the MEN1 consensus r egion they may play a role in sporadic endocrine tumors independent of the MEN1 gene or in other tumors, such as breast cancer, that have lo ss of heterozygosity within this region. (C) 1997 Academic Press.