RELN(RL-ALB2), AN ALLELE OF REELER ISOLATED FROM A CHLORAMBUCIL SCREEN, IS DUE TO AN IAP INSERTION WITH EXON SKIPPING

The reeler Albany2 mutation (Reln(rl-Alb2)) in the mouse is an allele of reeler isolated during a chlorambucil mutagenesis screen, Homozygou s animals had drastically reduced concentrations of reelin mRNA, in wh ich an 85-nt exon was absent, At the genomic level, the mutation was s hown to be due to an intracisternal A-particle insertion leading to ex on skipping, This appears to be the first observation of retrotranspos on insertion during chlorambucil mutagenesis. (C) 1997 Academic Press.