Measurement of biodegradable substances with a mycelia-sensor based on thesalt tolerant yeast Arxula adeninivorans LS3

The microbial sensor based on budding cells of the dimorphic yeast Arxula a deninivorans LS3 is one of the best suitable sensors for rapid measurement of biodegradable substances. However, Amnia is able to change its morpholog y (budding cells double left right arrow mycelia) in response to the cultiv ation temperature. The suitability of such mycelia was tested as microbial sensor component. The mycelia-based sensor possess a similar linear respons e, stability, limit of determination and detection compared with the conven tional budding cell-based sensor. However, differences exist in the storage capacity and substrate specificity: the mycelia-sensor has with six months a better storage stability as the conventional sensor, achieves a higher s ensitivity for specific amino acids or sugars, shows a better correlation b etween sensorBOD and the BOD5-value with yeast extract as model wastewater and is more suitable for measurement of salt water. (C) 2000 Elsevier Scien ce S.A. All rights reserved.