White matter injury in spinal cord ischemia - Protection by AMPA/kainate glutamate receptor antagonism

Background and Purpose-Spinal cord ischemia is a serious complication of su rgery of the aorta. NMDA receptor activation secondary to ischemia-induced release of glutamate is a major mechanism of neuronal death in gray matter. White matter injury after ischemia results in long-tract dysfunction and d isability. The AMPA/kainate receptor mechanism has recently been implicated in white matter injury. Methods-We studied the effects of AMPA/kainate receptor blockade on ischemi c white matter injury in a rat model of spinal cord ischemia. Results-Intrathecal administration of an AMPA/kainate antagonist, 6-nitro-7 -sulfamoyl-O-quinoxaline-2,3-dione (NBQX), 1 hour before ischemia reduced l ocomotor deficit, based on the Basso-Beattie-Bresnahan scale (0=total paral ysis; 21=normal) (sham: 21+/-0, n=3; saline: 3.7+/-4.5, n=7; NBQX: 12.7+/-7 .0, n=7, P<0.05) 6 weeks after ischemia. Gray matter damage and neuronal lo ss in the ventral horn were evident after ischemia, but no difference was n oted between the saline and NBQX groups. The extent of white matter injury was quantitatively assessed, based on axonal counts, and was significantly less in the NBQX as compared with the saline group in the ventral (sham: 10 63 +/-44/200x200 mu m, n=3; saline: 556+/-104, n=7; NBQX: 883 +/- 103, n=7) , ventrolateral (sham: 1060+/-135, n=3; saline: 411+/-66, n=7; NBQX: 676+/- 122, n=7), and corticospinal tract (sham: 3391+/-219, n=3; saline: 318+/-23 , n=7; NBQX: 588+/-103, n=7) in the white matter on day 42. Conclusions-Results indicate severe white matter injury in the spinal cord after transient ischemia. NBQX, an AMPA/kainate receptor antagonist, reduce d ischemia-induced white matter injury and improved locomotor function.