Promoters from glycolytic genes are widely used for gene overexpression in
the yeast Saccharomyces cerevisiae. Wine strains are not an exception, and
genes of enological interest have been expressed in this way. However, the
transcriptional pattern of glycolytic genes has never been studied during w
ine fermentation. In this work we describe the levels and expression patter
ns of glycolytic genes for a wine yeast strain during the alcoholic ferment
ation of three different musts. Results show similar transcriptional patter
ns for all genes studied: maximal levels of mRNA at the exponential growth
stage, and a gradual decrease during the stationary phase, in accordance wi
th the fermentation rates. The glyceraldehyde 3-phosphate dehydrogenase gen
es reache the highest transcriptional levels during wine fermentation, simi
larly as previously described for laboratory strains and conditions.