genetic diversity of Poa annua L. populations collected from western Oregon
grass-seed fields was surveyed using 18 randomly amplified polymorphic DNA
(RAPD) markers. Markers from 1357 individual plants from 47 populations co
llected at three sampling dates (fall, winter, and spring) for 16 sites wer
e used to measure genetic diversity within and among populations. Site hist
ories varied from low to high herbicide selection pressure, and some sites
were subdivided by 3 years of differing post-harvest residue management. Ge
ne diversity statistics, simple frequency of haplotype occurrence, and anal
ysis of molecular variance (AMOVA) revealed the presence of significant var
iability in P. annua among sites, among collection dates within sites, and
within collection dates. Nei gene-diversity statistics and population-diffe
renriation parameters indicated that P. annua populations were highly diver
se. Mean Nei gene diversity (h) for all 47 populations was 0.241 and total
diversity (HT) was 0.245. A greater proportion of this diversity, however,
was within (H-S=0.209) rather than among (G(sr)=0.146) populations. When po
pulations were grouped by season of collection, within-group diversity was
H-S=0.241, while among-group diversity was G(sr)=0.017. When populations we
re grouped by site, within-group diversity was Hs=0.224, while among-group
diversity was G(sr)=0.087. The diversity among populations within season fo
r fall, winter, and spring collections was G(sr)=0.121, 0.142, and 0.133, r
espectively. Populations collected from fields with histories of high herbi
cide selection pressure showed low differentiation among collection dates,
with G(sr) as low as 0.016, whereas those collected from fields with low he
rbicide selection pressure showed greater differentiation among collection
dates, with GS, as high as 0.125. At high selection-pressure sites, populat
ions were also lower in gene diversity las low as h=0.155), while at low se
lection-pressure sites there was higher gene diversity las high as h=0.286)
. The site to site variability was greater For the high selection-pressure
sites (G(sr)=0.107 or 69% of the total among-population variance), while th
e season of germination variability was greater at sites of low herbicide-s
election pressure (G(sr)=0.067, or 70% of the total among-population varian
ce). High initial diversity coupled with a long-term re-supply of genotypes
from the seed bank must have been factors in maintaining the genetic diver
sity of this weed despite the intensive use of herbicides. Knowledge of the
genetic diversity of Willamette Valley P. annua should help in formulating
more effective strategies for managing this weed.