Comparative genomic hybridization (CGH) in dogs - Application to the studyof a canine glial tumour cell line

Recurrent chromosome aberrations are associated with many human cancers. De tailed cytogenetic analysis of tumours has benefited enormously from the de velopment of molecular cytogenetic techniques based on fluorescence in situ hybridization (FISH). Comparative genomic hybridization (CGH) is a recentl y developed FISH technique that allows a rapid and comprehensive identifica tion of imbalanced genomic material in tumour DNA. Comparative genomic hybr idisation has been used widely in human medicine to evaluate losses and gai ns of tumour DNA isolated from a variety of sources, including fresh sample s, cell-culture material and archival specimens, and has been instrumental in identifying sites in the human genome which contain genes involved in tu mour development and progression. This report describes the first applicati on of CGH in the dog, illustrated by the analysis of DNA isolated from a ca nine glial tumour cell line. (C) 2000 Harcourt Publishers Ltd.