Ta. Foglia et Kc. Jones, QUANTITATION OF NEUTRAL LIPID MIXTURES USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH LIGHT-SCATTERING DETECTION, Journal of liquid chromatography & related technologies, 20(12), 1997, pp. 1829-1838
Citations number
12
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A high performance liquid chromatographic (HPLC) method was developed
for quantifying reaction mixtures obtained from the lipase-catalyzed t
ransesterification of fats and oils. The reaction mixtures of interest
were composed of neutral lipid classes that include alkyl esters, fre
e fatty acids, triglycerides, 1,2- and 1,3-diglycerides, and 1 (2)-mon
oglycerides. The method, a modification of a literature procedure, use
s a binary mobile phase of hexane (A) and methyl-t-butyl ether (B) eac
h modified with acetic acid (0.4%) and a gradient elution profile that
reduced analyses' times by 25%. Lipid classes were measured by use of
an evaporative light scattering detector (ELSD). Precision of injecti
on and linearity of response of the ELSD over the range of sample amou
nts of interest were established for the lipid classes measured by use
of standards. The method was applied to the compositional analysis of
prospective biofuel alkyl esters prepared from fats, oils, and recycl
ed greases.