The effect of a single bout of exhaustive exercise on muscle carbohydrate and lipid metabolism in a rat model of type 2 diabetes mellitus

The aim of the present study was to estimate whether a single bout of exhau stive exercise influences the glycogen and triglyceride (TG) content in red and white: gastrocnemius muscle and in the liver of rats with experimental type 2 diabetes. Experiments were carried out on male Wistar rats fed from 8 to 11 weeks of age with isocaloric standard or high-fat diet (HFD) with a previous injection of low-dose of streptozotocin (STZ) or vehicle at 2 da ys of age (I, control group; II, HFD; III, STZ; IV, STZ + HFD). Group IV (S TZ + HFD) represents a model of type 2 diabetes. Basal liver glycogen was m arkedly lower in all the studied groups compared to controls. Glycogen conc entration after exercise fell significantly in the examined tissues in all groups in comparison to basal conditions. A significant TG accumulation in examined tissues was observed in all the studied groups in comparison to co ntrols. Exercise decreased tissue TG content in all the groups, but it rema ined significantly higher in the experimental groups vs, control. We conclu de that in this model of type 2 diabetes,a single bout of exercise reveals defective utilization of tissue carbohydrates and lipids.