Leishmania amastigotes have been detected in the peripheral blood smears of
Indian kala-azar patients mostly during night. It was, therefore, thought
worthwhile to find out whether such amastigotes could be shown in healthy s
ubjects who did not have any symptoms by history or signs on clinical exami
nation. Leishmania-stained blood smears of 450 asymptomatic healthy individ
uals residing in an endemic village in Bihar (India) were examined by oil-i
mmersion microscopy for the detection of amastigote, six people (1.3%) show
ed the presence of Leishmania amastigotes. Given the low sensitivity of a s
ingle smear examination it is likely that a much greater proportion of asym
ptomatic persons had parasitemia than the observed 1.3%. This finding is im
portant from the point of view of transmission of disease, as also for modi
fying the current control measures. (C) 2000 Elsevier Science B.V. All righ
ts reserved.