Coagulation factor XI is a contaminant in intravenous immunoglobulin preparations

A small number of thromboembolic events, including deep venous thrombosis a nd myocardial infarction, have been reported in patients receiving IVIG, Th ese events have primarily occurred in patients receiving high-dose IVIG and have been attributed to an increase in blood viscosity. To test the hypoth esis that a procoagulant might be present in IgG preparations, twenty-nine samples of intravenous immunoglobulin (IVIG) from eight different manufactu rers were assayed for procoagulant activity. Twenty-six of these samples sh ortened the clotting time of factor XI-deficient plasma, of these, fourteen samples had factor XI activities greater than 0.001 U/ml of normal pooled plasma. The remaining samples possessed less than 0.001 U/ml of normal plas ma activity. The procoagulant activity in these samples could be inhibited by an anti-factor XI polyclonal antibody, suggesting that the procoagulant activity was factor XI, The procoagulant activity increased in two samples after storage at 4 degrees C for 4 weeks, likely as a result of factor XIa autoactivation. Additionally, activity in some IVIG samples was able to dir ectly activate factor IX, indicating that activated factor XI was present i n these samples. Finally, the degree of factor XI(a) contamination in the s amples was correlated with the manufacturer, suggesting that variations in the manufacturing process or source plasma affect the level of factor XI in the IVIG product. Because addition of small amounts of factor XIa to plasm a can lead to production of significant amounts of thrombin, we suggest tha t factor XIa present in some IVIG preparations could contribute to the in v ivo risk of thrombosis after IVIG therapy. (C) 2000 Wiley-Liss, Inc.