Treatment of uterine fibroid tumors in a nude mouse model using high-intensity focused ultrasound

OBJECTIVE: The purpose of this study was to investigate the potential effic acy of high-intensity focused ultrasound for the treatment of uterine fibro id tumors in a nude mouse model. STUDY DESIGN: A total of 60 female athymic nude mice were inoculated subcut aneously with 3 to 5 x 10(6) ELT-3 cells, a uterine fibroid tumor cell line . Tumor size was monitored with transcutaneous caliper measurements. The hi gh-intensity focused ultrasound probe was a concave, single-element, high-p ower transducer that operated at a frequency of 3.5 MHz. The tumors were tr eated for 30 to 60 seconds using a high-intensity focused ultrasonic intens ity of 2000 W/cm(2), depending on the tumor size. RESULTS: A single high-intensity focused ultrasonic treatment resulted in a n average reduction in tumor volume of 91% within 1 month of the treatment. Histologic analysis of tumors treated with high-intensity focused ultrasou nd showed coagulation necrosis and nuclear fragmentation of tumor cells. CONCLUSION: High-intensity focused ultrasound effectively reduced uterine f ibroid tumor size in a nude mouse model. Further studies are needed to asse ss the in situ response of uterine fibroids to high-intensity focused ultra sonic treatment.