K. Sakurada et al., cis-9,10-methylenehexadecanoic acid inhibits contractility and actomyosin ATPase activity of guinea pig myocardium, BIOC BIOP R, 274(2), 2000, pp. 533-536
Citations number
30
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Superfusion with a cyclopropane fatty acid, cis-9,10-methylenehexadecanoic
acid (10-300 mu M), reduced the contractility of papillary muscle isolated
from guinea pigs in a dose-dependent manner. cis-9,10-Methylenehexadecanoic
acid also inhibited the Mg2+-ATPase activity of guinea pig papillary myoca
rdium by about 40% at 400 mu M. Since cis-9,10-methylenehexadecanoic acid 4
mu M inhibited the K+-EDTA-ATPase activity inherent in myosin's catalytic
activity by about 25%, the fatty acid was thought to interact with the cata
lytic center of the myosin molecule. (C) 2000 Academic Press.