Novel ELISA for the measurement of immunoreactive bisphenol A

In the present study, anti-BPA serum RY 504 with high titer was raised in a rabbit using BPA mono-glutaryl-KLH conjugate as immunogen. ELISA was devel oped using above anti BPA serum RY 504 in 1:5,000 dilution, BPA as standard and HRP labeled BPA (BPA monoglutaryl HRP) as labeled antigen. The assay r ange was from 0.5 ng/ml to 5,000 ng/ml of BPA. The range of the intra- and inter-assay coefficients of variation were 3.6 to 10.8 % and 6.3 to 8.4 %, respectively. Further, we compared BPA concentration obtained from the HPLC analysis of glucronidase-treated urine of human subjects ingested coffee f rom a commercial coffee-containing can, with IR-BPA measured directly by EL ISA using the same urine sample, which was not treated with glucronidase. T he results showed the positive linear correlation between both values, indi cating strongly that the present ELISA can be efficiently utilized for the measurement of IR-BPA in samples in the environment including urine and pla sma.