Successful large volume leukapheresis on a small infant allogeneic donor

We successfully performed a hematopoietic stem cell apheresis on the smalle st allogeneic donor reported to date, a 6.1 kg female. After placement of a dialysis catheter in the left femoral vein, the COBE Spectra was primed wi th one unit of paternal whole blood. Heparin and anticoagulant citrate dext rose, solution A (ACD-A) were slowly administered to the patient, Ionized c alcium levels were checked hourly and calcium gluconate was given for hypoc alcemia, Coagulation parameters were checked throughout the procedure, We c ollected 4.4 x 10(6) CD34(+) cells/kg (recipient), The donor tolerated the procedure well and was discharged the following day. Five months later, the child manifests no obvious late effects.