Megavesicles implicated in the rapid transport of intracisternal aggregates across the Golgi stack

Citation
A. Volchuk et al., Megavesicles implicated in the rapid transport of intracisternal aggregates across the Golgi stack, CELL, 102(3), 2000, pp. 335-348
Citations number
49
Categorie Soggetti
Cell & Developmental Biology
Journal title
CELL
ISSN journal
00928674 → ACNP
Volume
102
Issue
3
Year of publication
2000
Pages
335 - 348
Database
ISI
SICI code
0092-8674(20000804)102:3<335:MIITRT>2.0.ZU;2-R
Abstract
Engineered protein aggregates ranging up to 400 nm in diameter were selecti vely deposited within the cis-most cisternae of the Golgi stack following a 15 degrees C block. These aggregates are much larger than the standard vol ume of Golgi vesicles, yet they are transported across the stack within 10 min after warming the cells to 20 degrees C. Serial sectioning reveals that during the peak of anterograde transport, about 20% of the aggregates were enclosed in topologically free "megavesicles" which appear to pinch off fr om the rims of the cisternae. These megavesicles can explain the rapid tran sport of aggregates without cisternal progression on this time scale.