Investigation of catechin and acridine derivatives using voltammetric and fluorimetric DNA-based sensors

The preconcentration-differential pulse voltammetric detection scheme with a carbon paste electrode bulk phase modified with DNA was used for the inve stigation of behaviour and the determination of trace levels of catechin an d acridine derivatives. The effect of electrochemical activation of the ele ctrode and the DNA-type was examined. The results are compared with those o btained by fluorimetric measurement of the TO-PRO-3 dye : DNA complex in th e presence of analytes. With voltammetric biosensors, the detection limits are in the nmol l(-1) concentration region. Using competitive reagents, the intercalation of analytes to DNA is indicated.