High-throughput screening of enzyme libraries

Directed evolution is becoming a widely used technique for modifying or enh ancing protein performance. Ultimately, the success of directed protein evo lution experiments hinges on the efficiency of the methods used to screen l ibraries for mutants with properties of interest. Although there is still a paucity of general methods for enzyme library screening, in recent years a number of promising strategies have emerged and are increasingly being use d to explore challenging issues in protein engineering.