Effects of 4-nonylphenol on sex differentiation and puberty in mosquitofish (Gambusia holbrooki)

Three days post-parturition mosquitofish were exposed to different concentr ations of 4-NP following a semi-static protocol. Exposure lasted up to the development of male anal fin in male individuals of the control group. Expo sure to 50 mu g/L 4-NP resulted in 100% females considering secondary sexua l characters, while external sex-ratio did not statistically differed from unity in control group. In group exposed to 0.5 and 5.0 mu g/L sex-ratio di d not differ from unity but incompletely developed gonopodium was observed in several individuals. Individuals exposed to 50 mu g/L 4-NP exhibited fem ale or undeveloped gonads, while gonadal sex-ratio did not statistically di ffer from unity in control group. Percentage of undeveloped gonads increase d with 4-NP concentration. Additional observations demonstrated hepatic his topathology in fish exposed to the highest concentration and growth reducti on dependent on 4-NP concentration. In a complementary experiment, extensiv e metabolism of [H-3]4-n-NP was characterized following in vivo exposure of juvenile mosquitofish suggesting that metabolism could modulate 4-NP toxic ity. This study suggests susceptibility of early life stages of mosquitofis h to endocrine modulators with regard to development of reproductive capabi lities.