Cytochrome c ' from Chromatium vinosum on gold electrodes

Cytochrome c' from Chromatium vinosum (CVCP) was immobilized at a surface-m odified gold electrode. Characterization of the CVCP electrode showed a qua si-reversible, diffusionless electrochemical redox behavior of the surface adsorbed protein with a formal potential of - 128 +/- 5 mV vs. Ag/AgCl. The heterogeneous electron transfer rate constant of adsorbed CVCP was determi ned to be about 50 s(-1). Different immobilization strategies were compared . The interaction of the immobilized CVCP with nitric oxide (NO) was invest igated and applied for a primary amperometric detection of NO in solution. (C) 2000 Elsevier Science S.A. All rights reserved.