G. Honda et al., HETEROLOGOUS PROTEIN-PRODUCTION IN ACREMONIUM-CHRYSOGENUM - EXPRESSION OF BACTERIAL CEPHALOSPORIN-C ACYLASE AND HUMAN THROMBOMODULIN GENES, Bioscience, biotechnology, and biochemistry, 61(6), 1997, pp. 948-955
We have developed an efficient expression system for foreign genes in
Acremonium chrysogenum. After inserting the foreign gene between the p
hosphoglycerate kinase (PGK) promoter and a terminator derived from A,
chrysogenum, multiple copies of this expression unit are tandemly lig
ated into cosmids and the resultant cosmids are introduced into A, chr
ysogenum. We expressed Pseudomonas cephalosporin C acylase and a human
thrombomodulin mutant protein containing the fourth, fifth, and sixth
epidermal growth factor (EGF)-like structures (E456), The acylase act
ivity in the transformants obtained using our system was several times
higher than that in the transformants without the use of the system,
The acylase proteins expressed had enzymatic and immunochemical proper
ties identical to those of authentic acylase, The transformants with t
he expression plasmid for E456 secreted biologically active E456 prote
in into the culture medium, The amino terminal sequence of the purifie
d E456 was identical to that of recombinant E456 obtained using mammal
ian cells.