RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) FOR RAPID IDENTIFICATION OFTHE SPOILAGE BACTERIUM ALICYCLOBACILLUS-ACIDOTERRESTRIS

Authors
YAMAZAKI K OKUBO T INOUE N SHINANO H
Citation
K. Yamazaki et al., RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) FOR RAPID IDENTIFICATION OFTHE SPOILAGE BACTERIUM ALICYCLOBACILLUS-ACIDOTERRESTRIS, Bioscience, biotechnology, and biochemistry, 61(6), 1997, pp. 1016-1018
Citations number
14
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
61
Issue
6
Year of publication
1997
Pages
1016 - 1018
Database
ISI
SICI code
0916-8451(1997)61:6<1016:RAPD(F>2.0.ZU;2-Q
Abstract
RAPD (randomly amplified polymorphic DNA) analysis was developed for r apid identification of the spoilage bacterium, Alicyclobacillus acidot errestris. Three primers, Ba-10, F-61, and F-64, allowed adequate disc rimination between strains of A. acidoterrestris and related bacteria in RAPD analyses, As compared with the conventional method and a RAPD assay against the isolates from various environmental and food samples , the results obtained from the RAPD pattern were identical to the ide ntification by the conventional method, We suggest that the RAPD analy sis is a rapid and reliable technique to distinguish A. acidoterrestri s from other related bacteria.