DETERMINATION OF ENDOGENOUS PEPTIDES WITH IN-VITRO ACE INHIBITORY ACTIVITY IN NORMOTENSIVE HUMAN PLASMA BY THE FLUOROMETRIC HPLC METHOD

Authors
MATSUI T MATSUFUJI H KAWASAKI T OSAJIMA Y
Citation
T. Matsui et al., DETERMINATION OF ENDOGENOUS PEPTIDES WITH IN-VITRO ACE INHIBITORY ACTIVITY IN NORMOTENSIVE HUMAN PLASMA BY THE FLUOROMETRIC HPLC METHOD, Bioscience, biotechnology, and biochemistry, 61(6), 1997, pp. 1052-1054
Citations number
15
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
61
Issue
6
Year of publication
1997
Pages
1052 - 1054
Database
ISI
SICI code
0916-8451(1997)61:6<1052:DOEPWI>2.0.ZU;2-Y
Abstract
An in vitro degradation test of angiotensin (ANG) II or III in normote nsive supine human plasma from 9 healthy male subjects confirmed the p roduction of smaller ANG metabolites with angiotensin I-converting enz yme inhibitory activity, These metabolites were identified as ANG (3-8 ), ANG (4-8), ANG (5-8), and ANG (3-4), whose respective peptide conce ntrations were determined by our proposed naphthalene-2,3-dialdehyde ( NDA)-HPLC method to be 64 +/- 9, 39 +/- 5, 176 +/- 22, and 197 +/- 35 fmol/ml of plasma.