Overexpression of T-type calcium channels in HEK-293 cells increases intracellular calcium without affecting cellular proliferation

Increased expression of low voltage-activated T-type Ca2+ channels has been correlated with a variety of cellular events including cell proliferation and cell cycle kinetics. The recent cloning of three genes encoding T-type alpha(1) subunits, alpha(1G), alpha(1H) and alpha(11), non allows direct as sessment of their involvement in mediating cellular proliferation, By overe xpressing the human alpha(1G) and alpha(1H) subunits in human embryonic kid ney (HEK-293) cells, we describe here that. although T-type channels mediat e increases in intracellular Ca2+ concentrations, there is no significant c hange in bromodeoxyuridine incorporation and flow cytometric analysis. Thes e results demonstrate that expressions of T-type Ca2+ channels are not suff icient to modulate cellular proliferation of HEK-293 cells. (C) 2000 Federa tion of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.