Fast, non-toxic, and inexpensive n-butanol preparation of recombinant plasmids

Various commercial and non-commercial plasmid preparation protocols are cur rently available. However, the kits are expensive and many of the protocols contain toxic chemicals. Here we present a novel, optimized and, therefore , very advantageous plasmid preparation protocol using n-butanol. The prepa ration can be performed quickly and no toxic chemicals are used, at overall costs of about one cent per plasmid preparation.