G. Henrion et al., IDENTIFICATION OF MATERNAL TRANSCRIPTS THAT PROGRESSIVELY DISAPPEAR DURING THE CLEAVAGE PERIOD OF RABBIT EMBRYOS, Molecular reproduction and development, 47(4), 1997, pp. 353-362
In order to characterize the changes that occur in the population of m
aternally inherited transcripts before the transition from maternal to
zygotic control of embryonic development (MZT) in mammals, we used ra
bbit embryos where zygotic transcription becomes necessary only after
the fourth cleavage division, during the second day that follows ferti
lization. In the present work we have associated mRNA differential dis
play and an RT-PCR based-method that allows amplification of the whole
population of messengers to identify and characterize maternal transc
ripts which are degraded throughout this early period of development.
While there is no major degradation of the polyA RNA population before
MZT we identify 4 transcripts which progressively disappear up until
the 8-16 cell stage. We also show that the degradation of one of these
maternal messengers is controlled by zygotic transcription, which is
not the case for the three others. This messenger shows homology with
the human FGF9 gene and is potentially a good candidate to address the
question of the molecular control of maternal to zygotic transition i
n early mammalian embryogenesis. (C) 1997 Wiley-Liss, Inc.