Dj. Groves et Ba. Morris, Veterinary sources of nonrodent monoclonal antibodies: Interspecific and intraspecific hybridomas, HYBRIDOMA, 19(3), 2000, pp. 201-214
The generation of monoclonal antibodies from species other than rats and mi
ce has developed slowly over the last 20 Sears. The advent of antibody engi
neering and realization of the advantages of nonmurine antibodies, in terms
of their superior affinities and specificities, and their potential as com
ponents of human and veterinary therapeutics has increased their relevance
recently. There have been significant advances in the development of myelom
a and heteromyeloma fusion partners. This is an opportune moment to consoli
date experiences of MAb production across the range of species of veterinar
y interest and place it into context with other developments in the field o
f monoclonal antibodies. The background to the development of antibodies fr
om species other than the mouse is discussed. The species and antigens used
to date are reviewed, as are the methods and results reported. A suggested
protocol is provided for first attempts to exploit the huge potential of t
his aspect of hybridoma technology and suggestions are made for its further
expansion.