Evaluation of the natural immunity in pups inoculated with a modified-livecanine parvovirus type 2b (CPV-2b) strain

Three pups 2-4 months old were vaccinated subcutaneously with the modified live canine parvovirus, CPV-2b/29-97 strain. During an observation period o f two weeks pups remained clinically health, exhibiting a vigorous post-vac cinal active serological response (haemoagglutinating inhibiting antibody t iters for CPV-2 ranging from 1:2560 to 1:5120 at 21 days post inoculation). Phagocytosis and killing of Candida albicans exerted by polymorphonuclear cells and monocytes did not undergo significant modifications 3-6 days post vaccination up to 30 days. Antibacterial activity mediated by peripheral b lood lymphocytes (Salmonella typhi was used as a target) was slightly, but not significantly decreased 3 days post vaccination. Conclusively, in pups the CPV type 2b vaccine seems to be safe as far as na tural immune responses are concerned, while its immunogenicity is preserved .