Differentiation of smoked Salmo salar, Oncorhynchus mykiss and Brama raii using the nuclear marker 5S rDNA

Authors
Carrera, E Garcia, T Cespedes, A Gonzalez, I Fernandez, A Asensio, LM Hernandez, PE Martin, R
Citation
E. Carrera et al., Differentiation of smoked Salmo salar, Oncorhynchus mykiss and Brama raii using the nuclear marker 5S rDNA, INT J FOOD, 35(4), 2000, pp. 401-406
Citations number
37
Categorie Soggetti
Food Science/Nutrition
Journal title
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
ISSN journal
09505423 → ACNP
Volume
35
Issue
4
Year of publication
2000
Pages
401 - 406
Database
ISI
SICI code
0950-5423(200008)35:4<401:DOSSSO>2.0.ZU;2-T
Abstract
PCR amplification of 5S rDNA was applied to differentiate smoked samples of Atlantic salmon (Salmo salar), rainbow trout (Oncorhynchus mykiss) and bre am (Brama raii). Contiguous divergent primers for the conserved coding regi on of the 5S rRNA gene allowed species-specific amplification of tandemly a rranged units of the 5S rDNA in these three related species. Thus, the 5S r DNA genetic marker may become very useful for inspection programmes intende d to assess the species identity of smoked products.