Determination of ginsenosides in Panax ginseng roots by liquid chromatography with evaporative light-scattering detection

A liquid chromatographic method was developed and validated for the determi nation of ginsenosides in Panax ginseng roots by using evaporative light-sc attering detection. Eighteen ginsenosides were separated on a reversed-phas e C-18 column with water-ammonium acetate-acetonitrile as the mobile phase. The method is suitable for the routine determination of ginsenosides in P, ginseng roots and extracts. The validation of the method was comprehensive for efficiency and recovery optimization of the P. ginseng roots extractio n, specificity by liquid chromatography/mass spectrometry, linearity, stabi lity, reproducibility, repeatability, intermediate precision, and robustnes s.