Assay for Laccase activity by microcalorimetry: Laccase was extracted fromChina lacquer of Rhus vernicifera

Authors
Wang, TZ Li, WP Wan, HW Zhou, PJ Qu, SS
Citation
Tz. Wang et al., Assay for Laccase activity by microcalorimetry: Laccase was extracted fromChina lacquer of Rhus vernicifera, J BIOCH BIO, 45(1), 2000, pp. 57-63
Citations number
18
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
ISSN journal
0165022X → ACNP
Volume
45
Issue
1
Year of publication
2000
Pages
57 - 63
Database
ISI
SICI code
0165-022X(20000810)45:1<57:AFLABM>2.0.ZU;2-L
Abstract
The reactions between Laccase (extracted from China lacquer of Rhus vernici fera) and various substrates (3,4-Dihydroxybenzaldehyde, Guaiacol, Pyrogall ol, Gallic acid) have been studied using LKB-2107 batch microcalorimetry sy stem. Based on calorimetry, a new method has been proposed. Laccase activit y and the Michaelis constant K-m have been determined simultaneously by thi s method. The method is simple, sample-saving, and valid for a wider range of substrate concentrations. Furthermore, it can be extended for assaying o ther enzymes catalyzing reactions using this method. (C) 2000 Elsevier Scie nce B.V. All rights reserved.