A FREE-RADICAL IN HUMAN CEREBROSPINAL-FLUID INVESTIGATED BY EPR

We have detected a free radical in human cerebrospinal fluid (CSF) and have identified as ascorbyl radical using electron paramagnetic reson ance (EPR). EPR spectrum of the radical in CSF was compared with one i n the corresponding serum. Determination of the ascorbyl radical conce ntrations in CSF and serum was attempted using known concentrations of a nitroxyl radical. Moreover, an alternative method to measure the bi ological radical was provided.