Control of bisecting GlcNAc addition to N-linked sugar chains

In the present study, experimental control of the formation of bisecting Gl cNAc was investigated, and the competition between beta-1,4-GalT (UDP-galac tose N-acetyl-glucosamine beta-1,4-galactosyltransferase) and GnT-III (UDP- N-acetylglucosamine:beta-D-mannoside beta-1,4-N--acetylglucosaminyltransfer ase) was examined. We isolated a beta-1,4-GalT-I single knockout human B ce ll clone producing monoclonal IgM and several transfectant clones that over expressed beta-1,4-GalT-I or GnT-III. In the beta-1,4-GalT-I-single knockou t cells, the extent of bisecting GlcNAc addition to the sugar chains of IgM was increased, where beta-1,4-GalT activity was reduced to about half that in the parental cells, and GnT-III activity was unaltered. In the beta-1,4 -GalT-I, transfectants, the extent of bisecting GlcNAc addition was reduced although GnT-III activity was not altered significantly. In the GnT-III tr ansfectants, the extent of bisecting GlcNAc addition increased along with t he increase in levels of GnT-III activity. The extent of bisecting GlcNAc a ddition to the sugar chains of IgM was significantly correlated with the le vel of intracellular beta-1,4-GalT activity relative to that of GnT-III. Th ese results were interpreted as indicating that beta-1,4-GalT competes with GnT-III for substrate in the cells.