Antibody-based biosensor for breast cancer with ultrasonic regeneration

We describe a novel method and instrumental setup for regenerating antibodi es immobilized on a fiberoptic probe of an immunosensor using ultrasonic ir radiation with broadband imaging transducers. The instrumental setup and ir radiation conditions for antibody regeneration using ultrasound are describ ed. The results of the measurements with antibody against breast cancer ant igen illustrate the effectiveness and potential of the regenerable immunose nsor. A 65% removal of the antigens bound to the Mab immobilized on the fib er surface is attained after ultrasound regeneration. (C) 2000 Society of P hoto-Optical Instrumentation Engineers. [S1083-3668(00)00703-6].